The Technion Researchers Find to NanoParticles may Threaten Heart

Nanoparticles, extremely tiny particles measured in billionths of a meter, are increasingly everywhere, and especially in biomedical products. Their toxicity has been researched in general terms, but now a team of Israeli scientists has for the first time found that exposure nanoparticles (NPs) of silicon dioxide (SiO2) can play a major role in the development of cardiovascular diseases when the NP cross tissue and cellular barriers and also find their way into the circulatory system. Their study is published in the December 2014 issue of Environmental Toxicology.

Prof. Michael Aviram
The research team was comprised of scientists from the Technion Rappaport Faculty of Medicine, Rambam Medical Center, and the Center of Excellence in Exposure Science and Environmental Health (TCEEH).

“Environmental exposure to nanoparticles is becoming unavoidable due to the rapid expansion of nanotechnology,” says the study’s lead author, Prof. Michael Aviram, of the Technion Faculty of Medicine, “This exposure may be especially chronic for those employed in research laboratories and in high tech industry where workers handle, manufacture, use and dispose of nanoparticles. Products that use silica-based nanoparticles for biomedical uses, such as various chips, drug or gene delivery and tracking, imaging, ultrasound therapy, and diagnostics, may also pose an increased cardiovascular risk for consumers as well.”

In this study, researchers exposed cultured laboratory mouse cells resembling the arterial wall cells to NPs of silicon dioxide and investigated the effects. SiO2 NPs are toxic to and have significant adverse effects on macrophages. a type of white blood cell that take up lipids, leading to atherosclerotic lesion development and its consequent cardiovascular events, such as heart attack or stroke. Macrophages accumulation in the arterial wall under atherogenic conditions such as high cholesterol, triglycerides, oxidative stress – are converted into lipids, or laden “foam cells” which, in turn, accelerate atherosclerosis development.

“Macrophage foam cells accumulation in the arterial wall are a key cell type in the development of atherosclerosis, which is an inflammatory disease” says co-author Dr. Lauren Petrick. “The aims of our study were to gain additional insight into the cardiovascular risk associated with silicon dioxide nanoparticle exposure and discover the mechanisms behind Si02’s induced atherogenic effects on macrophages. We also wanted to use nanoparticles as a model for ultrafine particle (UFP) exposure as cardiovascular disease risk factors.”

Both NPs and UFPs can be inhaled and induce negative biological effects. However, until this study, their effect on the development of atherosclerosis has been largely unknown. Here, researchers have discovered for the first time that the toxicity of silicon dioxide nanoparticles has a “significant and substantial effect on the accumulation of triglycerides in the macrophages,” at all exposure concentrations analyzed, and that they also “increase oxidative stress and toxicity.”

A recent update from the American Heart Association also suggested that “fine particles” in air pollution leads to elevated risk for cardiovascular diseases. However, more research was needed to examine the role of “ultrafine particles” (which are much smaller than “fine particles”) on atherosclerosis development and cardiovascular risk.

“The number of nano-based consumer products has risen a thousand fold in recent years, with an estimated world market of $3 trillion by the year 2020,” conclude the researchers. “This reality leads to increased human exposure and interaction of silica-based nanoparticles with biological systems. Because our research demonstrates a clear cardiovascular health risk associated with this trend, steps need to be taken to help ensure that potential health and environmental hazards are being addressed at the same time as the nanotechnology is being developed.

The Technion-Israel Institute of Technology is a major source of the innovation and brainpower that drives the Israeli economy, and a key to Israel’s renown as the world’s “Start-Up Nation.” Its three Nobel Prize winners exemplify academic excellence. Technion people, ideas and inventions make immeasurable contributions to the world including life-saving medicine, sustainable energy, computer science, water conservation and nanotechnology. The Joan and Irwin Jacobs Technion-Cornell Institute is a vital component of Cornell NYC Tech, and a model for graduate applied science education that is expected to transform New York City’s economy.

American Technion Society (ATS) donors provide critical support for the Technion—more than $1.95 billion since its inception in 1940. Based in New York City, the ATS and its network of chapters across the U.S. provide funds for scholarships, fellowships, faculty recruitment and chairs, research, buildings, laboratories, classrooms and dormitories, and more.

Source: ATS

Adults Sought for Study on Aging and Mobility

                                                               Walking stickman
The Biomechanics Laboratory in the kinesiology department is recruiting volunteers for a study about the effects of age and exercise on walking and muscle function. The researchers hope to learn about how changes in muscle function with age are related to the onset of disability.

The lab is looking for individuals who meet the following criteria: ages 55-70 with healthy body weight who participate in fewer than five 30-minute bouts of exercise per week (or less than a total of 150 minutes of planned exercise per week), no history of reconstructive surgery of the legs, no major health issues (heart disease, diabetes, neurological disease), able to walk for 30 minutes and no contraindications to MRI (metal implant, claustrophobia).

The study consists of two visits: a 1-hour visit to an MRI facility in Amherst and one 3-hour visit to the Biomechanics Lab on campus. During the lab visit researchers will collect data on how participants’ joints move as they walk over the ground and on a treadmill. The study will also collect data on the strength of the muscles in participants’ thighs. All procedures are non-invasive.

Source: UMass

Gene discovered that reduces risk of stroke

Lab microscope (stock image). The discovery of a gene that protects people against one of the major causes of stroke could lead to new treatments and prevention strategies for the disease.
Credit: © 18percentgrey / Fotolia

Scientists have discovered a gene that protects people against one of the major causes of stroke in young and middle-aged adults and could hold the key to new treatments.

Researchers from Royal Holloway, University of London, together with an international team from across the United States and Europe, have found that people with a specific variant of a gene, known as PHACTR1, are at reduced risk of suffering cervical artery dissection, which is caused by a tear in an artery that leads to the brain.

The new discovery, published in the journal Nature Genetics, could lead to new treatments and prevention strategies for the disease, which is a major cause of stroke in young adults. The same gene variant has also been identified as a protector against migraines and affects the risk of heart attack.

Professor Pankaj Sharma, from the School of Biological Sciences at Royal Holloway, said: "This is an important breakthrough. Our findings provide us with a greater understanding of how this region of the genome appears to influence key vascular functions, which could have major implications for the treatment of these severe and disabling conditions. "

In the largest study of its kind ever undertaken, researchers from around the world screened the entire genome of 1,400 patients with cervical artery dissection, along with 14,400 people without the disease. Cervical artery dissection can lead to compression of adjacent nerves and to blood clotting, potentially causing blockage of vessels and brain damage.

Professor Sharma, Professor of Clinical Neurology at Royal Holloway, added: "Further genetic analyses and worldwide collaborations of this kind provide hope of pinpointing the underlying mechanisms that cause stroke. The Bio-Repository of DNA in Stroke (BRAINS) study I am leading is creating a large stroke DNA biobank which will give an exciting opportunity to identify the genes directly linked to the condition."

Source: University of Royal Holloway London

Shape of things to come in platelet mimicry

By mimicking the shape, size, flexibility and surface chemistry of real platelets, artificial platelets are pushed out of the main blood flow to vessel walls. There, the surface chemistry enables them to anchor on damaged cells and induce faster clotting at the site. Credit: Anirban Sen Gupta

Artificial platelet mimics developed by a research team from Case Western Reserve University and University of California, Santa Barbara, are able to halt bleeding in mouse models 65 percent faster than nature can on its own.

For the first time, the researchers have been able to integratively mimic the shape, size, flexibility and surface chemistry of real blood platelets on albumin-based particle platforms. The researchers believe these four design factors together are important in inducing clots to form faster selectively at vascular injury sites while preventing harmful clots from forming indiscriminately elsewhere in the body.

The new technology, reported in the journal ACS Nano, is aimed at stemming bleeding in patients suffering from traumatic injury, undergoing surgeries or suffering clotting disorders from platelet defects or a lack of platelets. Further, the technology may be used to deliver drugs to target sites in patients suffering atherosclerosis, thrombosis or other platelet-involved pathologic conditions.

Anirban Sen Gupta, an associate professor of biomedical engineering at Case Western Reserve, previously designed peptide-based surface chemistries that mimic the clot-relevant activities of real platelets. Building on this work, Sen Gupta now focuses on incorporating morphological and mechanical cues that are naturally present in platelets to further refine the design.

"Morphological and mechanical factors influence the margination of natural platelets to the blood vessel wall, and only when they are near the wall can the critical clot-promoting chemical interactions take place," he said.

These natural cues motivated Sen Gupta to team up with Samir Mitragotri, a professor of chemical engineering at UC Santa Barbara, whose laboratory has recently developed albumin-based technologies to make particles that mimic the geometry and mechanical properties of red blood cells and platelets.

Together, the team has developed artificial platelet-like nanoparticles (PLNs) that combine morphological, mechanical and surface chemical properties of natural platelets.

The researchers believe this refined design will be able to simulate natural platelet's ability to collide effectively with larger and softer red blood cells in systemic blood flow. The collisions cause margination -- pushing the platelets out of the main flow and closer to the blood vessel wall -- increasing the probability of interacting with an injury site.

The surface coatings enable the artificial platelets to anchor to injury-site-specific proteins, von Willebrand Factor and collagen, while inducing the natural and artificial platelets to aggregate faster at the injury site.

Testing in mouse models showed that intravenous injection of these artificial platelets formed clots at the site of injury three times faster than natural platelets alone in control mice.

The ability to interact selectively with injury site proteins, as well as the ability to remain mechanically flexible like natural platelets, enables these artificial platelets to safely ride through the smallest of blood vessels without causing unwanted clots.

Albumin, a protein found in blood serum and eggs, is already used with cancer drugs and considered a safe material. Artificial platelets that don't become involved in a clot and continue to circulate are metabolized within one to two days.

The researchers believe the new artificial platelet design may be even more effective in larger volume blood flows where margination to the blood vessel wall is more prominent. They expect to soon begin testing those capabilities.

This research was previously funded by American Heart Association and is currently funded by National Institutes of Health.

In addition to stemming bleeding, Sen Gupta believes the technology could also be useful in delivering clot-busting medicines directly to clots, to treat heart attack or stroke without having to systemically suspend the body's coagulation mechanism. The artificial platelets may also be used to deliver cancer medicines to metastatic tumors that have high platelet interactions. Sen Gupta is seeking grants to pursue that work.

Source: Case Western Reserve University

Platelet-like particles augment natural blood clotting for treating trauma

Associate Professor Tom Barker and Research Scientist Ashley Brown examine bacteria growing on a plate, part of a technique for evolving antibodies in their work on platelet-like particles.
Credit: Georgia Tech Photo

A new class of synthetic platelet-like particles could augment natural blood clotting for the emergency treatment of traumatic injuries -- and potentially offer doctors a new option for curbing surgical bleeding and addressing certain blood clotting disorders without the need for transfusions of natural platelets.

The clotting particles, which are based on soft and deformable hydrogel materials, are triggered by the same factor that initiates the body's own clotting processes. Testing done in animal models and in a simulated circulatory system suggest that the particles are effective at slowing bleeding and can safely circulate in the bloodstream. The particles have been tested with human blood, but have not undergone clinical trials in humans.

Supported by the National Institutes of Health, the U.S. Department of Defense, and the American Heart Association, the research will be reported September 7, 2014, in the journal Nature Materials. Researchers from the Georgia Institute of Technology, Emory University, Children's Healthcare of Atlanta and Arizona State University collaborated on the research.

"When used by emergency medical technicians in the civilian world or by medics in the military, we expect this technology could reduce the number of deaths from excessive bleeding," said Ashley Brown, a research scientist in the Georgia Tech School of Chemistry and Biochemistry and first author of the paper. "If EMTs and medics had particles like these that could be injected and then go specifically to the site of a serious injury, they could help decrease the number of deaths associated with serious injuries."

The bloodstream contains proteins known as fibrinogen that are the precursors for fibrin, the polymer that provides the basic structure for natural blood clots. When they receive the right signals from a protein known as thrombin, these precursors polymerize at the site of the bleeding. The synthetic platelet-like particles use the same trigger, and so are activated only when the body's natural clotting process is initiated.

To create that trigger, the researchers followed a process known as molecular evolution to develop an antibody that could be attached to the hydrogel particles to change their form when they encounter thrombin-activated fibrin. The resulting antibody has a high affinity for the polymerized form of fibrin and a low affinity for the precursor material.

"Fibrin production is on the back end of the clotting process, so we feel that it is a safer place to try to interact with it," said Tom Barker, an associate professor in the Wallace H. Coulter Department of Biomedical Engineering at Georgia Tech and Emory University, and one of the paper's co-corresponding authors. "The specificity of this material provides a very important advantage in triggering clotting at just the right time."

The effectiveness of the platelet-like particles has been tested in an animal model and in a microfluidic chamber designed to simulate conditions within the body's circulatory system. In the chamber, tubes about the thickness of a human hair were lined with endothelial cells as in natural blood vessels.

The chamber was used to study normal human blood, as well as human blood that had been depleted of its natural platelets. In platelet-rich blood, clots formed as expected, and blood without platelets did not form clots. When the platelet-like particles were added to the platelet-depleted blood, it was able to clot.

The researchers also tested blood from infants that had undergone open heart surgery, which requires that their blood be diluted, reducing its clotting ability. When platelet-like particles were added to the dilute neonate blood, it was able to form clots.

Finally, safety testing was done on blood from hemophiliac patients. Because that blood lacks the triggers needed to cause fibrin formation, the particles had no effect. Before they can be used in humans, the particles will have to undergo human trials and receive clearance from the U.S. Food & Drug Administration (FDA).

About one micron in diameter, the particles were originally developed to be used on the battlefield by wounded soldiers, who might self-administer them using a device about the size of a smartphone. But the researchers believe the particles could also reduce the need for platelet transfusions in patients undergoing chemotherapy or bypass surgery, and in those with certain blood disorders.

"For a patient with insufficient platelets due to bleeding or an inherited disorder, physicians often have to resort to platelet transfusions, which can be difficult to obtain," said Dr. Wilbur Lam, another of the paper's co-authors and a physician in the Aflac Cancer and 
Blood Disorders Center at Children's Healthcare of Atlanta and the Department of Pediatrics at the Emory University School of Medicine. "These particles could potentially be a way to obviate the need for a transfusion. Though they don't have all the assets of natural platelets, a number of intriguing experiments have shown that the particles help augment the clotting process."

In addition to providing new treatment options, the particles could also cut costs by reducing costly natural transfusions, said Lam, who is also an assistant professor in the Coulter Department of Biomedical Engineering at Georgia Tech and Emory University.

What ultimately happens to the hydrogel particles circulating in the bloodstream will be the topic of future research, noted Brown. Particles of similar size and composition are normally eliminated from the body.

While the platelet-like particles lack many features of natural platelets, the researchers were surprised to find one property in common. Clots formed by natural platelets begin to contract over a period of hours, beginning the body's repair process. Clots formed from the synthetic particles also contract, but over a longer period of time, Brown noted.

Source: Georgia Institute of Technology

Neck manipulation may be associated with stroke

Vertebral artery as it passes through the neck vertebrae of the spine and enters the skull base. Arrows indicate head movement during lateral rotation and lateral flexion, motions that may be performed as part of a neck manipulation. Credit: © 2013 Trial FX.

Manipulating the neck has been associated with cervical dissection, a type of arterial tear that can lead to stroke. Although a direct cause-and-effect link has not been established between neck manipulation and the risk of stroke, healthcare providers should inform patients of the association before they undergo neck manipulation.

Treatments involving neck manipulation may be associated with stroke, though it cannot be said with certainty that neck manipulation causes strokes, according to a new scientific statement published in the American Heart Association's journal Stroke.

Cervical artery dissection (CD) is a small tear in the layers of artery walls in the neck. It can result in ischemic stroke if a blood clot forms after a trivial or major trauma in the neck and later causes blockage of a blood vessel in the brain. Cervical artery dissection is an important cause of stroke in young and middle-aged adults.

"Most dissections involve some trauma, stretch or mechanical stress," said José Biller, M.D., lead statement author and professor and chair of neurology at the Loyola University Chicago Stritch School of Medicine. "Sudden movements that can hyperextend or rotate the neck -- such as whiplash, certain sports movements, or even violent coughing or vomiting -- can result in CD, even if they are deemed inconsequential by the patient."

Although techniques for cervical manipulative therapy vary, some maneuvers used as therapy by health practitioners also extend and rotate the neck and sometimes involve a forceful thrust.

There are four arteries that supply blood to the brain: the two carotid arteries on each side of the neck, and the two vertebral arteries on the back of the neck. The influence of neck manipulation seems more important in vertebral artery dissection than in internal carotid artery dissection.

"Although a cause-and-effect relationship between these therapies and CD has not been established and the risk is probably low, CD can result in serious neurological injury," Biller said. "Patients should be informed of this association before undergoing neck manipulation."

The association between cervical artery dissection and cervical manipulative therapies was identified in case control studies, which aren't designed to prove cause and effect. An association means that there appears to be a relationship between two things, i.e., manipulative therapy of the neck and a greater incidence of cervical dissection/stroke. 

However, it's not clear whether other factors could account for the apparent relationship.

The relationship between neck manipulation and cervical artery dissection is difficult to evaluate because patients who already are beginning to have a cervical artery dissection may seek treatment to relieve neck pain, a common symptom of cervical artery dissection that can precede symptoms of stroke by several days.

You should seek emergency medical evaluation if you develop neurological symptoms after neck manipulation or trauma, such as:

• Pain in the back of your neck or in your head;
• Dizziness/vertigo;
• Double vision;
• Unsteadiness when walking;
• Slurred speech;
• Nausea and vomiting;
• Jerky eye movements.

"Tell the physician if you have recently had a neck trauma or neck manipulation," Biller said. 

"Some symptoms, such as dizziness or vertigo, are very common and can be due to minor conditions rather than stroke, but giving the information about recent neck manipulation can raise a red flag that you may have a CD rather than a less serious problem, particularly in the presence of neck pain."

Source: American Heart Association

Diagnosis targets in primary care are misleading, unethical, UK experts say

Last month, there was public outcry at the news that GPs in England would be paid £55 for each case of dementia diagnosed.

Now come targets for six other conditions, including diabetes coronary heart disease, asthma and depression, writes Dr Martin Brunet, a GP in Surrey. "But the data on which they are based are flawed, and the approach incentivises potentially harmful overdiagnosis," he argues.

Every practice in England has been told its diagnosis rate for each condition, estimated from practice data and the expected prevalence, he explains. The intention is to exert pressure on general practitioners to increase diagnosis rates, but he believes the principles behind such a policy need to be questioned.

Brunet argues that applying error prone national prevalence data to an individual practice is problematic. Although attempts are made to account for local demographics, practices may be under pressure to "improve" diagnosis rates that are far better than the data would suggest, he warns.

He also questions the ethical implications for individual patients of unnecessary tests and treatments that "could do more harm than good" and divert resources away from people with symptoms.

Targets in healthcare always threaten to undermine trust in the doctor-patient relationship, says Brunet. "For this reason patients need to trust that the doctor will act solely in their best interests, unencumbered by competing interests."

"NHS England needs to hear the clear message from doctors and patients that setting targets for diagnosis is problematic, unscientific, and unethical," he argues. "Instead, it needs to trust doctors and their patients to know when to seek a diagnosis."

Source: BMJ-British Medical Journal

Scientist finds genetic wrinkle to block sun-induced skin aging

UBC researchers created a device to act as a tanning bed for mice. Credit: Image courtesy of University of British Columbia

A scientific team at UBC and Providence Health Care have genetically engineered mice with less wrinkled skin, despite repeated exposure to wrinkle-inducing ultraviolet (UV) light.

The findings, published last week in Aging Cell, raises hope for a drug that would block the activity of Granzyme B in certain places, and thus prevent the aging and deterioration of tissues that depend on collagen -- not just skin, but blood vessels and lung passages.

viDA Therapeutics, a company co-founded by David Granville, a professor in the Department of Pathology and Laboratory Medicine and a principal investigator in the Centre for Heart Lung Innovation of UBC and St. Paul's Hospital, is currently developing a Granzyme-B inhibitor based on technology licensed from UBC. The company plans to test a topically applied drug within two years on people with discoid lupus erythematosus, an autoimmune disease worsened by sunlight that can lead to disfiguring facial scarring. (The musician Seal has such a condition.)

If the drug proves effective in preventing lupus-related skin lesions, there is potential for a cosmetic product to prevent the normal, gradual aging of the skin, which is mostly caused by sun exposure. But the drug might also be used for life-threatening conditions, such as aneurysms and chronic obstructive pulmonary disease, caused by the breakdown of collagen and other proteins that provide structure to blood vessels and lung passages.

Background

A serendipitous discovery: Granville was investigating the role of Granzyme B in atherosclerosis and heart attacks. He and his team wanted to see if the blood vessels of mice lacking Granzyme B were more resistant to hardening and narrowing, which is a major cause of heart attacks in human. In the process, they discovered that such mice retained youthful-looking skin compared to the aged skin on normal mice.

The Canadian Institutes of Health Research (CIHR), Genome BC, and Mitacs financially support this research.

An experimental tanning bed: Granville's team constructed a device to simulate sun exposure on mice. Each mouse was put in a carousel that slowly turned under UV lamps, exposing them for three to four minutes, three times a week -- enough to cause redness, but not to burn. After 20 weeks of repetitive exposure, it became clear that the skin of mice lacking Granzyme B had aged much less -- and their collagen was more intact -- compared to the control groups.

Source: University of British Columbia

Technique for cardiovascular diagnostics shows promise

Researcher Elira Maksuti is involved in developing and testing a promising method for diagnosing atherosclerosis, or hardening of the arteries. Credit: Staffan Larsson

A new technique developed at Sweden's KTH Royal Institute of Technology shows promise for early diagnosis and treatment of cardiovascular disease.

Hardening of the arteries, or atherosclerosis, is a common disorder that occurs when fat, cholesterol, and other substances build up in the walls of arteries and form hard structures called plaques. The condition can lead to heart attacks and strokes.

To diagnose atherosclerosis, doctors today rely on ultrasonic grayscale images to visually assess vascular function and how very large arteries move. The less mobility, the more developed the case of atherosclerosis.

But it is an indirect measurement. Better diagnoses can be made when the stiffness of the blood vessels can be analysed, explains Elira Maksuti, a researcher at the Department of Medical Imaging Technology at KTH.

"You need a doctor who is an expert and has extensive experience in order to get a good diagnosis," she says.

But by combining the technologies of shear wave elastography and ultrasound, Maksuti and researcher Erik Widmanh, have developed an inexpensive and non-invasive method not only for checking the stiffness of blood vessels, but for analysing the type of plaque present in the artery.

Maksuit says their method not only offers a potentially more effective way to diagnose atherosclerosis, but the ultrasound technology that it relies on is less expensive -- and safer -- than other imaging alternatives, such as magnetic resonance imaging (MRI) or computed tomography (CT).

The technique was tested on artificial blood vessels, or "phantom" vessels, which allowed the researchers to experiment with not only vascular stiffness, but also pressure and flow.

Maksuti says that with the success of tests on these phantoms, the next step is testing the technique with blood vessels from pigs. "These tests also look very promising," she says.

"We see two major future applications before us," she says. "The first is to determine when a patient's blood vessels are becoming rigid, that is, when the atherosclerosis process begins.

"The second application is to be able to diagnose the type of calcification -- or plaque -- present in the blood vessel." Not all plaque is the same: it ranges from hard to soft. If the plaque is soft and has a thin, hard shell, it is more likely to come loose inside the blood vessel.

It's a difficult distinction to determine. But the information is critical in deciding whether to open the artery surgically. "Today it is rather a matter of guessing. A doctor cannot know," she says. And to complicate matters, such operations can also generate strokes.

Source: KTH The Royal Institute of Technology

Screening tools to detect lung, heart disease developed by two high school students

Two Michigan high school students, sisters Ilina and Medha Krishen, have developed screening tools using electronic stethoscopes to detect lung and heart disease. The sisters will present their findings at CHEST 2014 in Austin, Texas next week. Credit: The Krishen family

Two Michigan high school students, sisters Ilina and Medha Krishen, have developed screening tools using electronic stethoscopes to detect lung and heart disease. The sisters will present their findings at CHEST 2014 in Austin, Texas next week.

Ilina Krishen became aware of the dangers of smoking and chemical air pollution when she saw the effects of lung disease on family members. Curious to find a way to detect early lung damage in people exposed to noxious air pollutants, Ilina, a high school senior at Port Huron Northern High School in Michigan, developed a screening mechanism using an electronic stethoscope. An electronic stethoscope overcomes the problem of low sound levels by electronically amplifying body sounds, using an electromagnetic diaphragm that captures the diaphragm movement as an electrical signal.

Ilina recruited 16 smokers, 13 firefighters, and 25 nonsmokers for her test. The electronic stethoscope recorded one breath cycle from each volunteer. Frequency peaks were used to analyze the frequency distribution of breath sounds. Differences of peaks above 125 Hz were analyzed.

Ilina found that the number of peaks was significantly higher in smokers and firefighters, even if the firefighters were nonsmokers. She realized that although firefighters wear protective masks when fighting fires, they often do not wear masks when making a second check of the building after the fire is out. "The firefighters are exposed to many poisonous chemicals that remain in the air after the fire has gone out," said Ilina. "Screening with an electronic stethoscope may be able to detect early changes in lung function in individuals without symptoms of lung disease."

Medha Krishen, Ilina's sister and a junior at Port Huron Northern High School, also presented a study that used an electronic stethoscope to screen student athletes for hypertrophic cardiomyopathy (HCM).

Medha studied 13 individuals: 10 with a normal cardiac sports physical and three with a diagnosis for HCM. Heart sounds were recorded in 5-second periods while the athletes were lying down, standing, and after exercise. Frequency peaks of a frequency amplitude plot were analyzed. Studies showed a significant difference in the distribution of frequency peaks in the two groups between the lying down position and after exercise. Normal athletes showed a lower percentage of peaks above 131 Hz after exercise, while the athletes at risk showed a rise in frequency peaks following exercise.

"When I was in fifth grade, a family friend died after exercise, and I always wanted to learn more about how to prevent something like that happening," said Medha. "My study analyzing heart sound frequencies may be a useful technique that school staff could use to screen for HCM."

The sisters are both athletes -- Ilina is a varsity tennis player, and Medha is an accomplished figure skater -- and they take a personal interest in the health of athletes. They are also nonsmokers and hope to encourage others not to smoke. After Ilina completed her study and showed her study subjects the results of her tests "two or three of the smokers have quit smoking, and that makes me feel good," says Ilina.

Source: American College of Chest Physicians

Intelligent training with a fitness shirt and an e-bike

The FitnessSHIRT reads out physiological signals like pulse and breath continuously when worn. The interpreted data can be viewed on a smartphone or tablet PC, for example. Credit: © Fraunhofer IIS

Fabric manufacturers are experiencing a revolution at present: if clothing previously offered protection against the cold, rain, and snow, the trend now is toward intelligent, proactive, high-tech textiles like self-cleaning jackets, gloves that recognize toxins, and ski anoraks with integrated navigational devices to make life easier for those wearing them.

Most clever clothing is only at the prototype stage. It is by no means off-the-rack yet. Soon the FitnessSHIRT from the Fraunhofer Institute for Integrated Circuits IIS in Erlangen, Germany, will be ready for the mass market. It continuously measures physiological signals such as breathing, pulse, and changes in heart rate -- metrics of adaptability and stress load. The intelligent sports shirt is expected to be available sometime in the next year, as an investor is already on-board.

Smart electronics are hidden in the material

Conductive textile electrodes integrated into the shirt's material capture the wearer's cardio activity. In addition, an elastic band around the upper body senses the motion of the chest during breathing. A removable electronic unit attached with snaps digitizes the raw data and calculates additional parameters like pulse rate or breath rate with the help of algorithms. The data are transmitted via radio link to a smartphone or optionally to a PC, where they are evaluated further and can be stored. These parameters form the basis for judging vital functions like stress, performance, exertion, or relaxation.

"The FitnessSHIRT can be employed a number of ways. It offers completely new options for the pursuit of sports, leisure activities, and wellness, as well as options for the medical branch," says Christian Hofmann, an engineer at IIS. For example, it could act as a training partner to provide seniors or rehabilitation patients with feedback on their vital signs during exercises or bicycling, and protect them from overexertion. Athletes will also benefit: for one thing, the SHIRT is more comfortable to wear than a chest strap. For another, the integrated sensors deliver more detailed information. Besides pulse and respiration, accelerometers sense the movement of the user and carry out an analysis. "If the pulse rate is high, for example, while the breath rate and the exercise activity is low, it could be a sign of possible heart problems," according to Hofmann.

The developers of the MENTORbike are also persuaded by the high degree of comfort when worn and the possibilities for performance diagnostics. MENTORbike is a new type of training device consisting of a pedelec, a smartphone, and an intelligent user service site on the internet. The project partners, led by BitifEye Digital Test Solutions, want to use the pedelec in combination with the FitnessSHIRT from IIS in future. The SHIRT will have a wireless connection via smartphone to the pedelec and the user service site on the internet, where the data can be viewed, analyzed, and documented. The smartphone mounted on the bicycle handlebars collects the vital parameters it receives like pulse and breath rate as well as the physical data, for instance the energy expended and the speed, analyzes them, and cuts in the electric motor as needed.

"If the pulse rate exceeds a maximum value of 150, for example, the rider is supported by the motor taking some of the load. If the pulse rate falls below a value of 80 beats per minute, the electric motor is throttled back and the pedal loading increased again. The motor output adapts automatically to the fitness of the cyclist," explains Markus Gratzfeld, an engineer with BitifEye. In this way, users are assured of an optimal level of training at all times, with neither over- nor under-exertion. Rehabilitation patients, especially persons with cardiovascular disease, could monitor their performance limits better, exercise more confidently, and increase their range of movement.

Source: Fraunhofer-Gesellschaft

A medical lab for the home

Microchip for the electrochemical detection of markers. Credit: © Fraunhofer FIT

Fraunhofer FIT demonstrates a mobile wireless system that monitors the health of elderly people in their own homes, using miniature sensors. Besides non-invasive sensors this platform integrates technology to take a blood sample and to determine specific markers in the patient's blood. At its core is the home unit, a compact device located in the patient's home. It incorporates the necessary software as well as sensors and the analytical equipment.

For years, cardiac diseases have been the most important cause of death globally. Mobile assistance systems that monitor vital parameters, e.g. blood pressure or heart rate, of risk patients in their homes could make their lives safer and more satisfying. A platform supporting this kind was developed and tested by researchers from Fraunhofer FIT, the Berlin Charité, T-Systems and several international partners.

Besides non-invasive sensors this platform integrates technology to take a blood sample and to determine specific markers in the patient's blood while the patient is at home. At its core is the home unit, a compact device located in the patient's home. It incorporates the necessary software as well as sensors and the analytical equipment. Wearable sensors for measuring vital parameters can be linked to the home unit, e.g. a pulse oximeter with a Bluetooth module in the patient's ear or a blood pressure monitor that sends its data to the system via WLAN. Using a nanopotentiostat, an electrochemical sensor, the system can measure the patient's glucose, lactate or cholesterol level. In addition, a fluorescence sensor using a laser diode captures the concentration of several cardiac markers.

To detect the risk-indicating markers in the blood, the patient uses a cartridge that she fills with a drop of blood from a prick in her finger. The cartridge is equipped with a microchip and also specially designed, so that the markers in the blood can be detected. "Miniaturized sensors in the home unit, which can detect traces of the markers down to the nano level, analyze the blood sample," says Professor Harald Mathis, head of the department 

'Biomolecular Optical Systems' of the Fraunhofer Institute for Applied Information 

Technology FIT.

The home unit aggregates the sensor data and sends the results to the patient's doctor or a medical center via secure Internet connection. A smartphone app presents the health data and the physician's feedback to the patient.

The system was developed by Fraunhofer FIT in cooperation with Charité and T-Systems Deutschland in the BMBF/EU-funded project Nanoelectronics for Mobile AAL Systems -- MAS.

Source: Fraunhofer-Institut fuer Angewandte Informationstechnik (FIT)

Wearable tech for battlefield, people at risk for heart attacks

The wearable system, under development by Sentient Science and the University at Buffalo, includes electrodes that relay data to a sensor, which connects with a remote computer network.
Credit: University at Buffalo

Wearable devices can count the steps you take and the calories you burn. But can they help soldiers in the field? Or prevent someone from having a heart attack?

Researchers at Sentient Science and the University at Buffalo say yes.

The sensor and software development company is working with UB engineering professor Albert H. Titus to create wearable technology that fuses real-time medical and physiological data with computer models. The system would then send personalized alerts indicating when the individual's level of stress, fatigue and resilience may put them in danger.

The work is funded by a $150,000 grant from the Office of Naval Research's Small Business Technology Transfer program, which enlists small businesses and research institutions to develop technology with military and commercial applications.

"Whether carrying 100 pounds of gear up a mountain or avoiding makeshift bombs, today's soldiers face incredible physical and mental stress," said Titus, PhD, professor and chair of UB's Department of Biomedical Engineering. "Our wearable system aims to measure how the body reacts to those challenges and combine that information with algorithms designed to help keep soldiers as safe as possible."

The wireless system, which is under development, will feature a series of electrodes that measure heart rate, brain activity and other vital signs. The electrodes may be attached to the skin like a Band-Aid or sewn into clothing that hugs the skin -- researchers are still deciding the best method.

The electrodes will relay information to a sensor (slightly smaller than a dime, attached to the skin like a patch), which will deliver that information to a remote computer network.

"It's like the hospital when you have a bunch of wires and equipment monitoring a patient. We're taking that technology and compressing it into a lightweight, wireless system," said the project's principal investigator Jennifer Haggerty, a research scientist and implementations manager at Sentient. Haggerty is an alumnus of UB.

As the information enters the computer network, it will fuse with Sentient's DigitalClone Live software, which has been validated by NASA and used to test the materials and components in the Hubble telescope, the F-35 fighter jet, wind turbines and other products.

The software includes complex algorithms that consider things like the terrain, weather and other environmental information, as well as the soldier's activity level. It will analyze the data and send personalized health alerts to soldiers and, if necessary, emergency medical facilities in the field. The idea is to improve soldiers' cognitive and physical abilities, making them more resilient and less prone to physical and psychological injuries.

In addition to serving the armed forces, the technology has commercial applications as a health-monitoring device. Sentient is exploring how the sensor can be applied to everyday items such as baseball caps. The individual wearing the cap would receive personalized health alerts regarding their risk of suffering a heart attack and other potential danger.

Source: University at Buffalo

Natural substance in red wine has an anti-inflammatory effect in cardiovascular diseases

Researchers see great therapeutic potential in the natural substance resveratrol, particularly in connection with prevention of the synthesis of inflammatory factors in cardiovascular diseases. Credit: photo/©: Peter Pulkowski, Mainz University Medical Center

A natural substance present in red wine, resveratrol, inhibits the formation of inflammatory factors that trigger cardiovascular diseases. This has been established by a research team at the Department of Pharmacology of the University Medical Center of Johannes Gutenberg University of Mainz (JGU) working in collaboration with researchers of the Friedrich Schiller University in Jena and the University of Vienna. Their results have recently been published in the scientific journal Nucleic Acids Research.

Despite the fact that they eat more fatty foods, the French tend to less frequently develop cardiac diseases than Germans. This so-called French Paradox is attributed to the higher consumption of red wine in France and it has already been the subject of various studies in the past. A number of research projects have actually demonstrated that the natural product resveratrol, present in red wine, has a protective effect against cardiovascular diseases. But what exactly is the reason for this? It seems that at least part of the protective effect can be explained by the fact that resveratrol inhibits the formation of inflammatory factors, a conclusion reached by the research team of Junior Professor Andrea Pautz and Professor Hartmut Kleinert of the Mainz University Medical Center following collaboration in a joint project with Professor Oliver Werz of the Friedrich Schiller University in Jena and Professor Verena Dirsch of the University of Vienna. In fact, the researchers discovered that the natural substance binds to the regulator protein KSRP and activates it. KSRP reduces the stability of messenger RNA (mRNA) in connection with a number of inflammatory mediators and thus inhibits their synthesis.

"We now know more precisely how resveratrol inhibits the formation of the inflammatory factors that trigger cardiovascular diseases. This is an important finding in view of the fact that more recent research has shown that cardiovascular diseases are significantly promoted by inflammatory processes in the body," said Pautz. Cardiovascular disorders, such as myocardial infarction and strokes, frequently occur in association with chronic inflammatory diseases, such as arthritis. The natural substance resveratrol thus has major therapeutic potential, particularly when it comes to the treatment of inflammatory diseases that can cause serious damage to the cardiovascular system.

Source: Universität Mainz